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p erk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p erk
    P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+erk1+2/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+XP+Rabbit+mAb/pmc13042271-96-58-63
    Average 99 stars, based on 13438 article reviews
    p erk - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction
    Article Snippet: .. The membranes were blocked with 5% skim milk and then incubated with primary antibodies against C5aR2 (sc-515734; Santa Cruz Biotechnology), PSS1 (ab157222; Abcam), PSS2 (ARP49961_P050; Aviva Systems Biology Corporation), MFN2 (12186-1-AP; Proteintech), XBP-1s (143F; BioLegend), p-EIF2α (28740-1-AP; Proteintech), EIF2α (11170-1-AP; Proteintech), CHOP (15204-1-AP; Proteintech), COX IV (11242-1-AP; Proteintech), calnexin (10427-2-AP; Proteintech), ERK1/2 (4695; Cell Signaling Technology), p-ERK1/2 (4370; Cell Signaling Technology), HA (ab9110, Abcam), FLAG (ab213519, Abcam), α-tubulin (HRP-80762; Proteintech), and β-actin (Ac028; ABclonal). .. The membranes were subsequently incubated with HRP-conjugated secondary antibodies (SA00001; Proteintech).

    Article Title: CAPG serves as a prognostic biomarker and promotes proliferation and migration in pancreatic ductal adenocarcinoma.
    Article Snippet: Equal amounts (20 μg) of total protein were separated by SDS-PAGE and transferred onto nitrocellulose membranes (Millipore, USA). .. Membranes were blocked with 5% non-fat milk and incubated overnight at 4°C with primary antibodies:CAPG (AF0013, Affinity Biosciences, China, 1:1000); p-ERK1/2 (4377s, CST, USA, 1:1000); ERK1/2 (5013s, CST, USA, 1:1000); Cyclin D1 (A19038, Abclonal, USA, 1:2000); β-actin (4970, CST, USA, 1:1000). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (S0001, Affinity Biosciences, China, 1:3000) at room temperature for 1 hour.

    Article Title: Asialoglycoprotein receptor 1 positively regulates PTGS2-induced inflammatory response in polycystic ovary syndrome via ERK1/2 pathway.
    Article Snippet: This study evaluated whether asialoglycoprotein receptor 1 (ASGR1) can regulate the expression of prostaglandin-endoperoxide synthase 2 (PTGS2) in granulosa cells (GCs) in polycystic ovary syndrome (PCOS).. The expression levels of ASGR1 in GCs and PCOS mice ovaries were assessed; to determine the in vivo role of ASGR1, it was overexpressed in the ovaries of female mice via adeno-associated virus injection, and RNA-sequencing analysis revealed that ASGR1 upregulated prostaglandin-endoperoxide synthase 2 (PTGS2).. Western blot confirmed the levels of activated ERK1/2 after ASGR1 overexpression, and ERK1/2 agonists and inhibitors were used to validate the downstream effects on PTGS2.

    Article Title: CAPG serves as a prognostic biomarker and promotes proliferation and migration in pancreatic ductal adenocarcinoma
    Article Snippet: Equal amounts (20 μg) of total protein were separated by SDS-PAGE and transferred onto nitrocellulose membranes (Millipore, USA). .. Membranes were blocked with 5% non-fat milk and incubated overnight at 4°C with primary antibodies:CAPG (AF0013, Affinity Biosciences, China, 1:1000); p-ERK1/2 (4377s, CST, USA, 1:1000); ERK1/2 (5013s, CST, USA, 1:1000); Cyclin D1 (A19038, Abclonal, USA, 1:2000); β-actin (4970, CST, USA, 1:1000). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (S0001, Affinity Biosciences, China, 1:3000) at room temperature for 1 hour.

    Membrane:

    Article Title: Asialoglycoprotein receptor 1 positively regulates PTGS2-induced inflammatory response in polycystic ovary syndrome via ERK1/2 pathway.
    Article Snippet: This study evaluated whether asialoglycoprotein receptor 1 (ASGR1) can regulate the expression of prostaglandin-endoperoxide synthase 2 (PTGS2) in granulosa cells (GCs) in polycystic ovary syndrome (PCOS).. The expression levels of ASGR1 in GCs and PCOS mice ovaries were assessed; to determine the in vivo role of ASGR1, it was overexpressed in the ovaries of female mice via adeno-associated virus injection, and RNA-sequencing analysis revealed that ASGR1 upregulated prostaglandin-endoperoxide synthase 2 (PTGS2).. Western blot confirmed the levels of activated ERK1/2 after ASGR1 overexpression, and ERK1/2 agonists and inhibitors were used to validate the downstream effects on PTGS2.

    other:


    Western Blot:

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses.
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_ 823588), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_ 390779), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_ 10622025), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029), and p-ASK1 (Cat#: 28846- 1-AP, 1: 1000, RRID: AB_2881220) were purchased from Proteintech (Wuhan, China).

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314 ), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000 ), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382 ), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626 ), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284 ), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369 ), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682 ), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090 ), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_823588 ), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373 ), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112 ), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_390779 ), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_10622025 ), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735 ), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292 ) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

    Immunofluorescence:

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses.
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_ 823588), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_ 390779), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_ 10622025), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029), and p-ASK1 (Cat#: 28846- 1-AP, 1: 1000, RRID: AB_2881220) were purchased from Proteintech (Wuhan, China).

    Article Title: Persistent Wnt/β-catenin signaling disables soft palatogenesis and palatal osteogenesis by inducing mesenchymal condensation
    Article Snippet: .. The primary antibodies against Lef1 (ab137827, Abcam, Cambridge, MA, United States), integrin ɑv (bs-1310R, Bioss Antibodies Inc., Beijing, China), ColII (K0921, Santa Cruz), Foxf1 (ab308633, Abcam), Dlx5 (10592-1-AP, Proteintech Group Inc., Wuhan, China), Runx2 (sc-101145, Santa Cruz Biotechnology Co., Ltd., Shanghai, China), Sox9 (ab185966, Abcam), Osterix (ab209484, Abcam), phospho-Smad1/5/8 (13820S, Cell Signaling Technology, Danvers, MA, United States), ɑ-SMA (ab7817, Abcam), ColI ( P28372 -BIF, Abmart, Shanghai, China), p-ERK1/2 (4370T, Cell Signaling Technology), Etv4 (10684-1-AP, Cloud-Clone Corp., Wuhan, China), Tbx15 (YN1399, Immunoway, Thermo Fisher Scientific, Shanghai, China), and Scx (ab307722, Abcam) were applied for immunofluorescence. .. The primary antibodies against Lef1 (ab137827, Abcam, Cambridge, MA, United States), integrin ɑv (bs-1310R, Bioss Antibodies Inc., Beijing, China), ColII (K0921, Santa Cruz), Foxf1 (ab308633, Abcam), Dlx5 (10592-1-AP, Proteintech Group Inc., Wuhan, China), Runx2 (sc-101145, Santa Cruz Biotechnology Co., Ltd., Shanghai, China), Sox9 (ab185966, Abcam), Osterix (ab209484, Abcam), phospho-Smad1/5/8 (13820S, Cell Signaling Technology, Danvers, MA, United States), ɑ-SMA (ab7817, Abcam), ColI ( P28372 -BIF, Abmart, Shanghai, China), p-ERK1/2 (4370T, Cell Signaling Technology), Etv4 (10684-1-AP, Cloud-Clone Corp., Wuhan, China), Tbx15 (YN1399, Immunoway, Thermo Fisher Scientific, Shanghai, China), and Scx (ab307722, Abcam) were applied for immunofluorescence.

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314 ), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000 ), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382 ), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626 ), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284 ), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369 ), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682 ), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090 ), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_823588 ), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373 ), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112 ), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_390779 ), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_10622025 ), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735 ), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292 ) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

    Ubiquitin Proteomics:

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses.
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_ 823588), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_ 390779), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_ 10622025), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029), and p-ASK1 (Cat#: 28846- 1-AP, 1: 1000, RRID: AB_2881220) were purchased from Proteintech (Wuhan, China).

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314 ), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000 ), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382 ), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626 ), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284 ), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369 ), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682 ), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090 ), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_823588 ), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373 ), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112 ), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_390779 ), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_10622025 ), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735 ), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292 ) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).



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    Overexpression of CRAF activates the RAF/MEK/ERK signaling pathway. Reverse transcription-quantitative PCR was used to analyze the mRNA expression of (A) ARAF, (B) BRAF, (C) CRAF, (D) MEK1, (E) MEK2, (F) ERK1and (G) ERK2. Western blotting was used to analyze the protein levels of (H) ARAF, (I) BRAF, (J) CRAF, (K) MEK1/2, (L) p-MEK1/2, (M) p-MEK1/2 to MEK1/2, (N) <t>ERK1/2,</t> (O) p-ERK1/2 and (P) p-ERK1/2 to ERK1/2. *P<0.05, **P<0.01 and ***P<0.001. p-, phosphorylated; OE, overexpression; NC, negative control; sh, short hairpin; ns, not significant; ARAF, A-Raf proto-oncogene serine/threonine-protein kinase; BRAF, B-Raf proto-oncogene serine/threonine-protein kinase; CRAF, C-Raf proto-oncogene serine/threonine-protein kinase.
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    Overexpression of CRAF activates the RAF/MEK/ERK signaling pathway. Reverse transcription-quantitative PCR was used to analyze the mRNA expression of (A) ARAF, (B) BRAF, (C) CRAF, (D) MEK1, (E) MEK2, (F) ERK1and (G) ERK2. Western blotting was used to analyze the protein levels of (H) ARAF, (I) BRAF, (J) CRAF, (K) MEK1/2, (L) p-MEK1/2, (M) p-MEK1/2 to MEK1/2, (N) <t>ERK1/2,</t> (O) p-ERK1/2 and (P) p-ERK1/2 to ERK1/2. *P<0.05, **P<0.01 and ***P<0.001. p-, phosphorylated; OE, overexpression; NC, negative control; sh, short hairpin; ns, not significant; ARAF, A-Raf proto-oncogene serine/threonine-protein kinase; BRAF, B-Raf proto-oncogene serine/threonine-protein kinase; CRAF, C-Raf proto-oncogene serine/threonine-protein kinase.
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    Image Search Results


    JP1 activates the Keap1-Nrf2-ARE pathway to regulate autophagic flux and temporally ameliorate oxidative stress in the spinal cords of SOD1-G93A transgenic mice. ( A ) Heatmap showing the expression profiles of Nrf2 signalling pathway-related genes in the spinal cord tissues of 120-day-old mice. The intensity indicates the gene expression level (red: high expression; blue: low expression). ( B–E ) Western blot analysis confirmed the protein levels of nuclear Nrf2 and cytoplasmic Keap1 in the spinal cord tissues of SOD1-G93A mice at different time points ( B ) and in those of WT mice, ALS model mice, and JP1-treated mice at 120 days ( D ). The results of the quantitative analysis ( C , E ) ( n = 3). ( F–I ) Protein levels of ERK1/2, p-ERK1/2, Cul3, HO-1, and NQO1 protein levels in the spinal cord tissues of the SOD1-G93A mice at different time points ( F ) and in those of the WT, SOD1-G93A , and JP1-treated mice at 120 days ( H ). The results of the quantitative analysis ( G , I ) ( n = 3). ( J-N ) RT‒qPCR analysis of Cul3 , Keap1 , Nfe2l2 , Nqo1 , and Hmox1 mRNA levels in WT, SOD1-G93A , and JP1-treated mice at 120 days ( n = 4). ( O-P ) Immunofluorescence detection of Nrf2 (green) and DAPI-stained nuclei (blue) in the spinal cord tissues of the model group/JP1-treated mice at different stages ( O ), and quantification of the Nrf2 nuclear/cytoplasmic fluorescence intensity ratio ( P ). Scale bar = 25 μm. * p < 0.05, ** p < 0.01, *** p < 0.001. NS = not significant ( p > 0.05)

    Journal: BMC Medicine

    Article Title: JP1 peptide modulates oxidative stress and autophagy via Keap1-Nrf2-ARE in ALS model mice

    doi: 10.1186/s12916-026-05119-w

    Figure Lengend Snippet: JP1 activates the Keap1-Nrf2-ARE pathway to regulate autophagic flux and temporally ameliorate oxidative stress in the spinal cords of SOD1-G93A transgenic mice. ( A ) Heatmap showing the expression profiles of Nrf2 signalling pathway-related genes in the spinal cord tissues of 120-day-old mice. The intensity indicates the gene expression level (red: high expression; blue: low expression). ( B–E ) Western blot analysis confirmed the protein levels of nuclear Nrf2 and cytoplasmic Keap1 in the spinal cord tissues of SOD1-G93A mice at different time points ( B ) and in those of WT mice, ALS model mice, and JP1-treated mice at 120 days ( D ). The results of the quantitative analysis ( C , E ) ( n = 3). ( F–I ) Protein levels of ERK1/2, p-ERK1/2, Cul3, HO-1, and NQO1 protein levels in the spinal cord tissues of the SOD1-G93A mice at different time points ( F ) and in those of the WT, SOD1-G93A , and JP1-treated mice at 120 days ( H ). The results of the quantitative analysis ( G , I ) ( n = 3). ( J-N ) RT‒qPCR analysis of Cul3 , Keap1 , Nfe2l2 , Nqo1 , and Hmox1 mRNA levels in WT, SOD1-G93A , and JP1-treated mice at 120 days ( n = 4). ( O-P ) Immunofluorescence detection of Nrf2 (green) and DAPI-stained nuclei (blue) in the spinal cord tissues of the model group/JP1-treated mice at different stages ( O ), and quantification of the Nrf2 nuclear/cytoplasmic fluorescence intensity ratio ( P ). Scale bar = 25 μm. * p < 0.05, ** p < 0.01, *** p < 0.001. NS = not significant ( p > 0.05)

    Article Snippet: The primary antibodies used included anti-JWA (1:100, Prof. Jianwei Zhou’s laboratory), αV (1:5000, ABclonal, A19071), β3 (1:2000, ABclonal, A19073), Nrf2 (1:1000, ABclonal, A0674), LC3B (1:2000, Abcam, ab48394), p62 (1:1000, Abcam, ab109012), HO-1 (1:5000, ABclonal, A1346), NQO1 (1:2000, ABclonal, A23486), Keap1 (1:500, ABclonal, A1820), ERK1/2 (1:5000, ABclonal, A4782), p-ERK1/2 (1:2000, ABclonal, AP0947), Cullin3 (1:1000, ABclonal, A16455), GAPDH (1:1000, Abcam, ab9485), β-actin (1:10,000, ABclonal, AC0126), and α-tubulin (1:5000, ABclonal, A6830) antibodies.

    Techniques: Transgenic Assay, Expressing, Gene Expression, Western Blot, Immunofluorescence, Staining, Fluorescence

    ML385 intervention validated the core role of the Nrf2 pathway in the therapeutic effects of ALS and JP1. ( A ) Kaplan‒Meier survival curves of the ALS model mice, ML385-treated mice, JP1-treated mice, and JP1 + ML385-cotreated mice ( n = 5). ( B-C ) Behavioural assessments of SOD1-G93A mice at day 120: rotarod test ( B ) and pole test ( C ) ( n = 10). ( D-E ) Representative Western blots ( D ) and quantitative analysis ( E ) of autophagy-related proteins p62 and LC3B in the spinal cord tissues of the indicated groups on day 120. ( F-G ) Representative immunofluorescence double-staining images ( F ) showing the localization of autophagosomes (LC3B, red) within neurons (NeuN, green) in the lumbar spinal cord and quantification of the mean LC3B fluorescence intensity within NeuN-positive cells ( G ). Scale bar = 25 μm. ( H-I ) Transmission electron microscopy images ( H ) showing mitochondrial ultrastructure and autophagosomes in spinal cord tissues at day 120, and quantification of abnormal mitochondria ( I ). Red arrows indicate damaged mitochondria; green arrows indicate autophagosomes, and blue arrows indicate lysosomes. Scale bars = 2 μm (overview) and 500 nm (enlarged view). ( J-K ) Representative Western blots ( J ) and quantitative analysis ( K ) of ERK1/2, p-ERK1/2, Cul3, HO-1, and NQO1 protein expression levels in the spinal cord tissues of the indicated groups at day 120. ( L-M ) Representative immunofluorescence images ( L ) showing subcellular localization of Nrf2 (green) in the spinal cord tissues, and quantification of the Nrf2 nuclear/cytoplasmic fluorescence intensity ratio ( M ). Scale bar = 25 μm. Data are presented as mean ± SEM, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. NS, not significant ( p > 0.05)

    Journal: BMC Medicine

    Article Title: JP1 peptide modulates oxidative stress and autophagy via Keap1-Nrf2-ARE in ALS model mice

    doi: 10.1186/s12916-026-05119-w

    Figure Lengend Snippet: ML385 intervention validated the core role of the Nrf2 pathway in the therapeutic effects of ALS and JP1. ( A ) Kaplan‒Meier survival curves of the ALS model mice, ML385-treated mice, JP1-treated mice, and JP1 + ML385-cotreated mice ( n = 5). ( B-C ) Behavioural assessments of SOD1-G93A mice at day 120: rotarod test ( B ) and pole test ( C ) ( n = 10). ( D-E ) Representative Western blots ( D ) and quantitative analysis ( E ) of autophagy-related proteins p62 and LC3B in the spinal cord tissues of the indicated groups on day 120. ( F-G ) Representative immunofluorescence double-staining images ( F ) showing the localization of autophagosomes (LC3B, red) within neurons (NeuN, green) in the lumbar spinal cord and quantification of the mean LC3B fluorescence intensity within NeuN-positive cells ( G ). Scale bar = 25 μm. ( H-I ) Transmission electron microscopy images ( H ) showing mitochondrial ultrastructure and autophagosomes in spinal cord tissues at day 120, and quantification of abnormal mitochondria ( I ). Red arrows indicate damaged mitochondria; green arrows indicate autophagosomes, and blue arrows indicate lysosomes. Scale bars = 2 μm (overview) and 500 nm (enlarged view). ( J-K ) Representative Western blots ( J ) and quantitative analysis ( K ) of ERK1/2, p-ERK1/2, Cul3, HO-1, and NQO1 protein expression levels in the spinal cord tissues of the indicated groups at day 120. ( L-M ) Representative immunofluorescence images ( L ) showing subcellular localization of Nrf2 (green) in the spinal cord tissues, and quantification of the Nrf2 nuclear/cytoplasmic fluorescence intensity ratio ( M ). Scale bar = 25 μm. Data are presented as mean ± SEM, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. NS, not significant ( p > 0.05)

    Article Snippet: The primary antibodies used included anti-JWA (1:100, Prof. Jianwei Zhou’s laboratory), αV (1:5000, ABclonal, A19071), β3 (1:2000, ABclonal, A19073), Nrf2 (1:1000, ABclonal, A0674), LC3B (1:2000, Abcam, ab48394), p62 (1:1000, Abcam, ab109012), HO-1 (1:5000, ABclonal, A1346), NQO1 (1:2000, ABclonal, A23486), Keap1 (1:500, ABclonal, A1820), ERK1/2 (1:5000, ABclonal, A4782), p-ERK1/2 (1:2000, ABclonal, AP0947), Cullin3 (1:1000, ABclonal, A16455), GAPDH (1:1000, Abcam, ab9485), β-actin (1:10,000, ABclonal, AC0126), and α-tubulin (1:5000, ABclonal, A6830) antibodies.

    Techniques: Western Blot, Immunofluorescence, Double Staining, Fluorescence, Transmission Assay, Electron Microscopy, Expressing

    Overexpression of CRAF activates the RAF/MEK/ERK signaling pathway. Reverse transcription-quantitative PCR was used to analyze the mRNA expression of (A) ARAF, (B) BRAF, (C) CRAF, (D) MEK1, (E) MEK2, (F) ERK1and (G) ERK2. Western blotting was used to analyze the protein levels of (H) ARAF, (I) BRAF, (J) CRAF, (K) MEK1/2, (L) p-MEK1/2, (M) p-MEK1/2 to MEK1/2, (N) ERK1/2, (O) p-ERK1/2 and (P) p-ERK1/2 to ERK1/2. *P<0.05, **P<0.01 and ***P<0.001. p-, phosphorylated; OE, overexpression; NC, negative control; sh, short hairpin; ns, not significant; ARAF, A-Raf proto-oncogene serine/threonine-protein kinase; BRAF, B-Raf proto-oncogene serine/threonine-protein kinase; CRAF, C-Raf proto-oncogene serine/threonine-protein kinase.

    Journal: Molecular Medicine Reports

    Article Title: RUVBL1 and CRAF promote periodontal ligament stem cell osteogenic differentiation via the MEK/ERK signaling cascade

    doi: 10.3892/mmr.2026.13881

    Figure Lengend Snippet: Overexpression of CRAF activates the RAF/MEK/ERK signaling pathway. Reverse transcription-quantitative PCR was used to analyze the mRNA expression of (A) ARAF, (B) BRAF, (C) CRAF, (D) MEK1, (E) MEK2, (F) ERK1and (G) ERK2. Western blotting was used to analyze the protein levels of (H) ARAF, (I) BRAF, (J) CRAF, (K) MEK1/2, (L) p-MEK1/2, (M) p-MEK1/2 to MEK1/2, (N) ERK1/2, (O) p-ERK1/2 and (P) p-ERK1/2 to ERK1/2. *P<0.05, **P<0.01 and ***P<0.001. p-, phosphorylated; OE, overexpression; NC, negative control; sh, short hairpin; ns, not significant; ARAF, A-Raf proto-oncogene serine/threonine-protein kinase; BRAF, B-Raf proto-oncogene serine/threonine-protein kinase; CRAF, C-Raf proto-oncogene serine/threonine-protein kinase.

    Article Snippet: The antibodies were as follows: GAPDH (1:1,000; cat. no. P30008M; Abmart Pharmaceutical Technology Co., Ltd.), A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) (cat. no. bs-2251R), CRAF (cat. no. bs-23170R), MEK1/2 (cat. no. bs-1041R), phosphorylated (p)-MEK1/2 (cat. no. bs-3270R), ERK1/2 (cat. no. bsm-33232M), p-ERK1/2 (cat. no. bs-3016R; all BIOSS), RUVBL1 (cat. no. 74775; Cell Signaling Technology, Inc.), ERK1/2 (all 1:500; cat. no. bsm-33232M; BIOSS), B-Raf proto-oncogene serine/threonine-protein kinase (BRAF) (1:2,000; cat. no. ab33899; Abcam) and HRP-conjugated universal secondary antibody [cat. nos.

    Techniques: Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Negative Control

    Overexpression of RUVBL1 activates the MEK/ERK signaling pathway. Reverse transcription-quantitative PCR was used to analyze the mRNA expression of (A) ARAF, (B) BRAF, (C) CRAF, (D) MEK1, (E) MEK2, (F) ERK1 and (G) ERK2. Western blotting was used to analyze protein levels of (H) ARAF, (I) BRAF, (J) CRAF, (K) MEK1/2, (L) p-MEK1/2, (M) p-MEK1/2 to MEK1/2, (N) ERK1/2, (O) p-ERK1/2 and (P) p-ERK1/2 to ERK1/2. *P<0.05, **P<0.01 and ***P<0.001. RUVBL1, RuvB-like AAA ATPase-1; p-, phosphorylated; OE, overexpression; NC, negative control; sh, short hairpin; ns, not significant; ARAF, A-Raf proto-oncogene serine/threonine-protein kinase; BRAF, B-Raf proto-oncogene serine/threonine-protein kinase; CRAF, C-Raf proto-oncogene serine/threonine-protein kinase.

    Journal: Molecular Medicine Reports

    Article Title: RUVBL1 and CRAF promote periodontal ligament stem cell osteogenic differentiation via the MEK/ERK signaling cascade

    doi: 10.3892/mmr.2026.13881

    Figure Lengend Snippet: Overexpression of RUVBL1 activates the MEK/ERK signaling pathway. Reverse transcription-quantitative PCR was used to analyze the mRNA expression of (A) ARAF, (B) BRAF, (C) CRAF, (D) MEK1, (E) MEK2, (F) ERK1 and (G) ERK2. Western blotting was used to analyze protein levels of (H) ARAF, (I) BRAF, (J) CRAF, (K) MEK1/2, (L) p-MEK1/2, (M) p-MEK1/2 to MEK1/2, (N) ERK1/2, (O) p-ERK1/2 and (P) p-ERK1/2 to ERK1/2. *P<0.05, **P<0.01 and ***P<0.001. RUVBL1, RuvB-like AAA ATPase-1; p-, phosphorylated; OE, overexpression; NC, negative control; sh, short hairpin; ns, not significant; ARAF, A-Raf proto-oncogene serine/threonine-protein kinase; BRAF, B-Raf proto-oncogene serine/threonine-protein kinase; CRAF, C-Raf proto-oncogene serine/threonine-protein kinase.

    Article Snippet: The antibodies were as follows: GAPDH (1:1,000; cat. no. P30008M; Abmart Pharmaceutical Technology Co., Ltd.), A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) (cat. no. bs-2251R), CRAF (cat. no. bs-23170R), MEK1/2 (cat. no. bs-1041R), phosphorylated (p)-MEK1/2 (cat. no. bs-3270R), ERK1/2 (cat. no. bsm-33232M), p-ERK1/2 (cat. no. bs-3016R; all BIOSS), RUVBL1 (cat. no. 74775; Cell Signaling Technology, Inc.), ERK1/2 (all 1:500; cat. no. bsm-33232M; BIOSS), B-Raf proto-oncogene serine/threonine-protein kinase (BRAF) (1:2,000; cat. no. ab33899; Abcam) and HRP-conjugated universal secondary antibody [cat. nos.

    Techniques: Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Negative Control